Translational_Unit

Part:BBa_K112005:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-09-03)


rbs.T4 holin!


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

PCR bx6/bx2 on Bjh1301		(704 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112005
----------------------------------------
bx6		Forward Biobricking of {rbs.T4 Holin!}
ccagtGAATTCatgAGATCTcttaaaaggagggtctatggcagcacctagaatatc
bx2		Reverse Biobricking of {rbs.T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc

References